Induction of apoptosis in HL-60 cells treated with the extract of lichen forming fungus, Hemithecium oryzaeforme (Fée) Staiger

Sung-Ju Lee, Kyung-Yun Kang, Yun-Ho Hwang, Min-Hye Jeong, Jae-Seoun Hur, Sung-Tae Yee

Abstract


Summary. The extract of lichen forming fungus, Hemithecium oryzaeforme and its components induced apoptotic effects to HL-60 cells and through increasing the sub-G1 population and by causing early and late apoptosis, as determined by Annexin V/PI staining. In addition, decreases in the mitochondrial membrane potential were observed in HL-60 cells treated with the extract. Several apoptotic features were observed including increased caspase-3, -8, -9 activities. These results provided evidence that the extract of lichen forming fungus, Hemithecium oryzaeforme induces apoptosis in human leukemia HL-60 cells.

Industrial relevance. Lichens, symbiotic organisms of fungi and algae, have been used for oriental medicine and have been reported to have a variety of biologically activities including anti-cancer activity. And, the induction of apoptosis in tumor cells has been used to a major focus of anti-cancer drug development. Therefore, because the extract of lichens was identified with apoptotic activity, can be provided as the potential mediator to induce specific apoptosis about HL-60.

Keywords. lichen forming fungus; Hemithecium oryzaeforme; apoptosis

Keywords


lichen forming fungus; Hemithecium oryzaeforme; apoptosis

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