Lactogenic study of the ethyl-acetate fraction of Hibiscus sabdariffa linn seed on pituitary prolactin level of lactating albino rats

I G Bako, A M Mabrouk, S M Abubakar, A Mohammed


Summary. The effect of ethyl-acetate fraction of Hibiscus sabdariffa l. seed on pituitary prolactin and milk production was evaluated in albino rats. Twenty four lactating rats were randomly grouped at parturition into control, metoclopramide-treated and Ethyl-acetate-treated group consist of six rats in each group (n=6). The lactating rats were administered control (normal saline), metoclopramide (5mg/kg) and ethyl-acetate fraction (100 and 200mg/kg) respectively from day 3-17 of lactation. Milk yield for rats were estimated by pup weight and weight gain. The animals were then euthanized on the day 18 and pituitary prolactin was analyzed using prolactin kit. The prolactin level of ethyl-acetate fraction of Hibiscus sabdariffa showed a significant increase (P<0.01) when compared to control group. Pup weight gain was also significantly higher (P<0.05) than the control group. This can be inferred that ethyl-acetate fraction of Hibiscus sabdariffa l. seed has lactogenic activity because it increases pituitary prolactin level and milk production in lactating female albino rats. The LD50 of Ethyl-acetate fraction Hibiscus sabdariffa l. was found to be above 5000mg/kg.

Industrial relevance. The outstanding advantage of this galactagogue option is that, it is safer, affordable and tolerable, and it is taken as an alternative in preference to anti-psychotic drugs that have side effect of drowsiness and depression. The plant calyces, leaves and seeds are eaten as foods because it contain substantial amount of essential fatty, Tocopherol (Vitamin E), ascorbic acid (Vitamin C), mineral salts calcium, magnesium, sodium, potassium and phosphorus.

Keywords. Hibiscus sabdariffa; prolactin; lactation; milk; pituitary


Hibiscus sabdariffa; prolactin; lactation; ethyl acetate; milk, pituitary.

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